Paper review of this article
With scheme Figure 2. Heterologousexpression and in vitro analysis of the cqsB genes. a) HPLC analysis
of the standard of 1 (i)and the culture extracts from S. lividans TK23:pSE-cqsB (ii), S. lividans
TK23:pSEcqsBDcqsB4 (iii), and S. lividans TK23:pSE101 (iv;negative control). b) HPLC analysis of the in
vitro coupling reaction of the CqsB1, CqsB2, and CqsB3 reaction mixture (i)and the CqsB1 and CqsB3
reaction mixture (ii). c) Proposed biosynthetic pathway of 1.
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