Challenges of using Volunteers in disaster response such as training, legal concerns, personal exposure, insurance, integration into ICS, etc.
Pig organ samples were collected from the slaughter-line after meat inspection in Finland, 16 M. avium positive pig organs (paper 2 and 3). 7 pig organs samples from Netherlands was kindly provided by Gerard Wellenberg (paper 4). Thirteen clinical M. avium isolates collected from Finnish human patients in 2001-2004 were randomly selected. The origin of the isolates was sputum, bronchial washings and lung biopsies (papers 2 and 3) M. avium strains from pigs and humans as well as reference strains were stored at the culture collection of Mycobacterial Reference Laboratory, National Public Health Institute in Turku, Finland. 3.2.Typing of mycobacteria Mycobacteria strains (16 pigs and 13 humans) were identified by direct sequence determination of 16S rRNA gene fragments and typed both by IS1245 restriction fragment length polymorphism (RFLP) and Variable-Number Tandem Repeat (VNTR) of genetic interspersed repetitive units of mycobacteria (MIRUs). Identification and RFLP and MIRU-VNTR typing were carried out in Mycobacterial reference laboratory, National Public Health institute in Turku, Finland. The discriminatory index (DI) of RFLP and MIRUs were calculated. 3.3. Mycobacteria specific real-time qPCR Description of the samples in real-time qPCR is shown in table 1. Sampl>GET ANSWER