Many would agree that advertisements are effective ways to market products and services. But does all that media saturation come with a cost? What does responsible advertising look like? Do advertisers have an ethical responsibility to society? Do they have an ethical responsibility to children? Why or why not? Historically, gender and gender roles have been pervasive in American culture. As such, this question asks you to first consider the binary terms, male and female. Can people in any gender role have both “feminine” and “masculine” characteristics? Do you see a danger in limiting people to one or the other?
Madin-Darby canine kidney strain I (MDCK-I) epithelial cell will be essential in carrying out this experiment because of the cultured components that contain Tjs like claudin-4 and claudin-1 (Stevenson et al., 1988). As stated earlier, importance of C-CPE’s is that they can degrade and bind onto target proteins. Meaning that when MDCK-I cells are incubated and bonded onto claudin -4, degradation will be apparent, thus disrupting the function of tight junctions. MDCK-I will be attained from the American Type Culture Collection and grown on a plate at a certain density (Sonoda et al., 1999). Cells will be measured by their conditions consistently and closely through a EVOM Epithelial Voltmeter. The TER will be measured after a Bx or CPE has been incorporated with the target cell for eight hours. This process will be given with various Bx doses for five times (Min et al., 2015). The same controls will be used to measure the paracellular pathway flux in addition to incorporating an impermeable membrane tracer in each control. Fluorescein isothiocyanate-dextrals (FITC-dextrans) from Sigma Chemical Company are the specific tracers that will be used as fluorescent probes to observe the strength of permeability. Before conducting this portion of the experiment, MDCK-I cells will be prepared the same way as mentioned earlier, but for 24 hours. This will allow 4k, 10k, and 40k masses to be dissolved fully in a P buffer. The 4k FITC-dextran that contains minimal amount of P buffer will be placed in the apical region, while the larger amount of P buffer will be placed in the basal region. This step will be repeated for the 10k and 40k masses to avoid bias and efficient results. A fluorometer will be used to check the intensity of the fluorescence that takes place after three hours (Sonoda et al., 1999). An unpaired t-test will be performed and if the results conclude in an increase of paracellular pathway flux and a decrease in TER, then this proves that the function of the tight junctions have been interfered.>GET ANSWER