Discuss how nurse managers and nurse leaders contribute to the reengineering of health care.
Denmark) according to the method described by Marshal12. Protein concentration was determined by the Kjeldahl method13. Water-Soluble Phenolic Content: The water-soluble phenolic content was measured via the Folin- Ciocalteu procedure, according to an assay modified by Shetty et al.15. Homogenized water extract, was prepared by the method of Apostolidis et al.16, and 1 ml was transferred into a test tube and mixed with 1 ml of 95 % ethanol and 5 ml of distilled water. To each sample, 0.5 ml of 50 % (V/V) Folin- Ciocalteu’s reagent was added and mixed. After 5 min, 1 ml of 5 % Na2CO3 was added to the reaction mixture and allowed to stand for 60 min. The absorbance was read at 725 nm in a spectrophotometer (Jenway, Model 6305, UV/Vis., England). The absorbance values were converted to water-soluble phenolics and were expressed in mg gallic acid equivalents per gram of dry matter of sample. Standard curves were established using various concentrations of gallic acid in water. Antioxidant Activity (AOA) by DPPH Radical Scavenging Assay: The capacity to scavenge the 2,2-diphenyl-1- picrylhydrazyl (DPPH) free radical was monitored according to the method reported by Apostolidis et al.16. To 3 ml of 60 μM DPPH in ethanol, 250 μl of each homogenized water extract was added, the decrease in absorbance was monitored at 517 nm in a spectrophotometer (Jenway, Model 6305, UV/Vis., England). DPPH scavenging effect was calculated as percentage of DPPH discoloration using the equation: %scavenging effect = [(ADPPH−AS)/ADPPH] ×100, where AS is the absorbance of the solution when the sample extract has been added at a particular level>GET ANSWER