Does MSC exosome increased polarization of M2 Macrophages promote functional recovery in rat SCI.
- What markers for M1 and M2 macrophages will you use?
- How do you identify these on M1 and M2 macrophages in the spinal cord? What reagents and tools will be used?
- What tools are used to observe that MSC exosomes are in M2 macrophages? How do you know that they are not in M1s?
- Identify one cytokine that is pro-inflammatory and one that is anti-inflammatory, which are secreted by M1 and M2 macrophages, respectively. See the clue below.
- How do you do a BBB score? What does it tell you?
- How do you measure white matter? What does it represent?
The clue to #4. Look up Laskin https://pubs.acs.org/doi/10.1021/tx900086v read the abstract and figure carefully.
these are large articles, thus you only need to highlight several sections that you plan to use.
Qu & Aang (2017) was carried out to establish how Mesenchymal Stem Cells (MSCs) influence the reparative actions in SCI.
Cheng &He (2017) established that Macrophages play a critical role in reactivating healing for secondary injuries.
Gennsel & Zhang (2015) provides a comparative analysis of the recovery and inflammatory reactions between SCI and tissues that achieves full wound resolution.
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