MSC exosome effects

Does MSC exosome increased polarization of M2 Macrophages promote functional recovery in rat SCI.

  1. What markers for M1 and M2 macrophages will you use?
  2. How do you identify these on M1 and M2 macrophages in the spinal cord? What reagents and tools will be used?
  3. What tools are used to observe that MSC exosomes are in M2 macrophages? How do you know that they are not in M1s?
  4. Identify one cytokine that is pro-inflammatory and one that is anti-inflammatory, which are secreted by M1 and M2 macrophages, respectively. See the clue below.
  5. How do you do a BBB score? What does it tell you?
  6. How do you measure white matter? What does it represent?

The clue to #4. Look up Laskin read the abstract and figure carefully.

these are large articles, thus you only need to highlight several sections that you plan to use.

Qu & Aang (2017) was carried out to establish how Mesenchymal Stem Cells (MSCs) influence the reparative actions in SCI.
Cheng &He (2017) established that Macrophages play a critical role in reactivating healing for secondary injuries.
Gennsel & Zhang (2015) provides a comparative analysis of the recovery and inflammatory reactions between SCI and tissues that achieves full wound resolution.

Sample Solution