• The goal of microscopy is to create a magnified image of objects too small to be seen with the eye alone.
• Brightfield microscopes use a combination of glass lenses and light to view the specimen.
• Using an oil immersion objective lens allows for higher magnification.
• Microscopes are often used to study bacteria, cells, and tissues.
• Fields of study where a microscope is a necessary tool include:
o + Cytology
o + Histology
o + Microbiology
o – Pathology
• In this simulation, you will learn how to correctly switch from the 40X high powered objective lens to the 100X oil immersion lens.
• Oil immersion is an essential technique for light microscopy. Used only on the 100x power objective, oil immersion is when a drop of immersion oil is added to the slide, contacting both the sample and objective. Without this oil, some of the peripheral light that passes through the specimen is scattered into air or the slide, decreasing resolution.
Before you begin
• Immersion oil can only be used with the 100X objective lens. The oil must be properly removed before using the 4X, 10X, or 40X objectives lenses.
• Your instructor may have a different specific protocol for cleaning the microscope objective lens and the slide than presented in this simulation.
• Knowledge of how to operate a brightfield microscope is assumed in this lab simulation.
• Phase 1: Use of immersion oil
- Focus on the cell in the slide with the yellow star using 4X objective. Use coarse and fine focus knobs to focus image
- Rotate the nosepiece to 10x. Focus image using the fine focus knob
- Rotate the nosepiece to 40x. Focus image using the fine focus knob
- Rotate nosepiece halfway toward 100X objective (green ring). Apply one drop of immersion oil to slide
- Rotate 100X lens into oil drop. Use fine focus knob to focus image
- Rotate nosepiece to blank space next to 4x objective. Using lens paper, remove immersion oil from 100X objective and microscope slide
• Phase 2: Save Lab Data
Write your lab report in essay format. Your report must include the following in 1-2 pages:
• Resources cited
ase study will address the concept of inflation – the rise in average level of prices sustained over time that corresponds to a fall in the internal (domestic) purchasing power of money – with regards to Venezuela. The goal here is to explore several trains of enquiry in order to critically evaluate the impact inflation has had – and may potentially have – on the national income and economic growth of Venezuela. As it stands, Venezuela’s inflation rate – 282972.8% – significantly exceeds that which holds 2nd position – Zimbabwe -175.66% (WorldEconomicForum 2019). In order to better decipher the notable disparity in inflation rates between Venezuela and the rest of the world, several areas will be analysed. This macroeconomic issue will be addressed with regards to challenges surrounding it alongside any potential benefits, it’s impact on the labour market, it’s fiscal impact in terms of taxes and government spending and potential policies that could be implemented in hope of combating it. Venezuela holds the highest recorded oil reserves in the world – possessing approximately 300 billion barrels – even surpassing Saudi Arabia. Evidently, oil is one of Venezuela’s most valuable commodities accounting for 95% of Venezuela’s exports and 25% of its gross domestic product (Independent 2018). However, during a period of time in which the global price of oil dropped, foreign demand to buy Venezuelan oil dipped simultaneously. A key factor that lead to Venezuela’s current crisis, is evidently their sole dependence on a single commodity – oil. As University of Florida’s Gamarra explains, this means “you are bound to the ups and downs of the oil price,”. Without a range of high value added assets, an economy lacks diversity and is vulnerable to ‘moments of downturns in your principal commodities (CNBC 2019).’ On an individual basis, hyperinflation renders any savings worthless due to its eroding impact on money. Consequently, people may hoard goods for instance, food due to the soaring prices. Situations such as these may lead to shortages of food supply, contributing to the issue further.>GET ANSWER