Evaluate the movie, The Matrix, in terms of the philosophical issues raised with (1) skepticism and (2) the mind-body problem.
loss and a large flow of intestinal fluid can occur when an active pore forms and disrupts the selective barrier. With the use of a transepithelial electrical resistance assay (TEER), it can reveal any specific disruptions and bacterial pathogens that increase paracellular flux (Spitz et al.,1995). As stated earlier, CPE damage causes food poisoning also known as a gratrointestinal disease; therefore, is the root cause of diarrhea and inflammation. It is important to know in this study how bacteria affects intestinal epithelium and how it is responsible for such diseases. Additionally, what steps are needed in order to prevent future occurring problems of food-borne pathogens. We hypothesize that bacteria is the source of inhibiting tight junction function by displacing occludins snf degrading claudins to form active proes, which in turn causes food poisoning symptoms. Experimental Design: The TER will be used to determine the function of TJ and paracellular pathway flux under three conditions: MFCK-I cells that are treated with C-CPE, MDCK-I cells treated with bacteria x (Bx), and lastly a baseline MDCK-I cell that gets no treatment. The significance of this method is to assay the plasma membrane epithelial resistance and functional barrier. A disrupted barrier in the tight junctions would be inefficient and will have very low resistance. Meanwhile the TER will measure how much resistance is occurring, the measurements of the paracellular pathway will be able to specify the size of molecules, especially larger ones, that are passing through the barriers (Stevenson et al., 1988). Madin-Darby canine kidney strain I (MDCK-I) epithelial cell will be essential in carrying out this experiment because of the cultured components that contain Tjs like claudin-4 and claudin-1 (Stevenson et al., 1988). As stated earlier, importance of C-CPE’s is that they can degrade and bind onto target proteins. Meaning that when MDCK-I cells are incubated and bonded onto claudin -4, degradation will be apparent, thus disrupting the function of tight junctions. MDCK-I will be attained from the American Type Culture Collection and grown on a plate at a certain density (Sonoda et al., 1999). Cells will be measured by their conditions consistently and closely through a EVOM Epithelial Voltmeter. The TER will be measured after a Bx or CPE has been incorporated with the target cell for eight hours. This process will be>GET ANSWER