a. In the treatment of carbon monoxide poisoning using hyperbaric oxygen, it is desirable to maintain an oxygen concentration of 0.1 mM in the patient’s interstitial fluid. What is the minimum necessary pressure of oxygen in the chamber to achieve this concentration if the patient is maintained at 37oC? You may assume that that interstitial fluid is a dilute aqueous solution. Carbon monoxide has a higher affinity for hemoglobin than oxygen so using higher oxygen pressures are necessary to displace CO from the hemoglobin molecule.
b. You now need to complete the electrolyte by adding NaCl (molar mass 58.4 g/mol) to the DMSO/water solution. You plan to place cells in the electrolyte and cool to -2.5oC retaining the liquid phase. What is the minimum quantity of NaCl, in grams, that must be added to 100 ml of the DMSO/water electrolyte to avoid rupturing the cells by expansion, if the intracellular fluid has an overall concentration of 0.2 M? The rupture strength of a cell membrane from internal pressure is 150 kPa.
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