Evaluate and compare the transfection methods of calcium phosphate, Lipofectamine 2000, and superfect on CHO- K1 cells using a pReceiver- M03 Expression Clone containing the genes for human insulin, green florescence protein, and antibiotic resistance to Geneticin. The cells are then subjected to clonal selection to isolate stable high producing clones through an examination of GFP expression levels, and the use of selective media containing G418 sulphate.
calcium phosphate, Lipofectamine 2000, and superfect
well as free health care. As the people and the petro-state rely on economic stability fueled by a steadily depleting resource they remove themselves from any glimpse of change and growth. They become largely removed from government corruption and waste as it has little impact on their pockets, in turn propagating their own moral hazard. The citizens attend school, yet, recently, they have graduated and remained unemployed for an extended period of time. If the available jobs on the market are not offered by the government, individuals find little to no incentive to take them. Instead, imported foreign workers from surrounding countries find themselves filling these jobs and sending the money back to their families. Therefore, the building of NEOM will more than likely succeed if the government strategically allocates money and diversifies their investments, however, the citizens of Saudi Arabia may contribute very little to this. Why work hard for your riyal when your father who humbly worked before you can give it to you with ease and the imported foreign workforce can sacrifice their energy and lives for their families and build the technological hub of the country’s dreams? Saudi Arabia may plan to avoid downfall by slowing its dependence on oil with the reforms of Vision 2030, but it does not mean the country will stop doing whatever it takes to have its way… and its oil; Saudi Arabia will continue to attempt use its political and oil leverage to shirk off the part they have played in the death of Khashoggi, blockade the people of Yemen, fight off the Houthis/Iranians, and gain control of the straits of Bab el-Mandeb over the course of the Yemeni Civil War. In the crusade for oil and p>GET ANSWER