Transfection methods of calcium phosphate

Evaluate and compare the transfection methods of calcium phosphate, Lipofectamine 2000, and superfect on CHO- K1 cells using a pReceiver- M03 Expression Clone containing the genes for human insulin, green florescence protein, and antibiotic resistance to Geneticin. The cells are then subjected to clonal selection to isolate stable high producing clones through an examination of GFP expression levels, and the use of selective media containing G418 sulphate.

calcium phosphate, Lipofectamine 2000, and superfect

Sample Solution

ACED ESSAYS