Rigorous discussion of how this method of solving the problem will benefit others.
Analysis of the effectiveness and efficiency of the chosen tool or technique, and discussion of other possible ways the problem could have been solved.
Contextualization and application of the chosen tool or process within professional practice.
Rigorous justification of the tool or process selected to address the problem, with support from the academic and professional literature.
Analysis of current tools available to solve the problem or improve professional practice, comparing and contrasting to identify benefits and issues.
Identification of an appropriate problem, issue, or question within the practice or application of the discipline.
No intro needed- the rest of the paper is completed.
Identify current policies to prevent the trafficking of human beings and propose improvements to existing policies.
The paper must answer the following questions:
- What impact does combating human trafficking at the local level have on national security?
- Does human trafficking increase our threat of terrorism?
- Will educating our citizens be the key to securing our nation?
- What proposed changes are necessary to law to stabilize the security of our nation and stop human trafficking?
ases (A) acetonitrile:ammonium formate (20 mM in 1% formic acid) (5:95 v/v) and (B) acetonitrile:ammonium formate (10 mM in 1% formic acid) (80:20 v/v) and were run according to previously described gradient program(Blessborn et al. 2010). The compounds were analyzed on a Tosoh ” 5 ”m C18 (150 mm ” 2 mm) column protected by a precolumn security guard C8 (8mm x2 mm) (Tosoh Bioscience, PA). The UV detector was monitored at 280nm. Data acquisition and quantification were performed using HystarTM and Data AnalysisTM (Bruker, Bremen, Germany). Estimation of dose intake time for SDX To estimate the probable timing of drug intake, we compared the whole blood concentrations of SDX at baseline (C0) and on Day 7 (C7) after a complete treatment with AS+SP for the same patients. Assuming a terminal elimination half-life (t”) of 7.2 days for SDX, an inter-individual variability of 30% and a similar dosage on pre-study exposure and during the study, a back-calculation was done to estimate the intake time of the drug before baseline sampling: Intake time =ln(C7/C0).t1/2/ln(2)+7[days] The variability on t” was used to estimate a 90% confidence interval around this intake time, considering plausible inter-individual variations in elimination rate (White et al. 1999). Similar calculation was not attempted for PYR because of its short half-life period (Hodel et al. 2009). Isolation of genomic DNA Parasites genomic DNA was extracted from clinical samples by using QIAamp DNA min kit (Qiagen, valencia, CA) according to the manufacturer’s protocol with slight modification. Pfdhfr and pfdhps gene products were amplified using earlier reported methods (Duarai singh et al., 1998) and then digested using restriction enzymes for analysing point mutations in Pfdhfr (codon 51, 59, 108 and 164), Pfdhps gene(436, 437, 540, 581 and 613) and pfcrt mutation analysis was done according to Vathsala et al 2004. Applied Biosystem thermocycler was used for all PCR amplification reactions. Digested PCR product (5-8 microlitre) was analysed on 1.5 % agarose gel containing ethidium bromide (0.5”g/ml) and 0.5X TBE running buffer (pH 8.0). Digested PCR products were visualized under UV transilluminator and digitally captured with the help of gel documentation system (Alpha Imager EP, USA). Molecular sizes of PCR fragments were calculated using gene tool (Alpha Inotech, version 22.214.171.124). Ethical clearance The study was approved by Scientific Advisory Committee and Research Advisory Committee of National Institute of Malaria Research (NIMR) and ethical clearance of by institution ethical committee. Statistical analysis>GET ANSWER