The diagram below shows the pattern of bands observed in an autoradiography after a DNA fragment was sequenced using the Sanger dideoxy method. In the figure below, the lanes are labeled according to the ddNTP added in the reaction.
A. (3 pts) What is the DNA sequence of the synthesized strand, the one producing the band pattern shown? Label the 5' and 3' ends. B. (3pts) What is the DNA sequence of the template strand that was used to produce the band pattern shown? Label the 5' and 3' ends. c. (4 pts) Write the sequence of the fragment produced if you prepare a sequencing reaction using a nucleotide mix containing only ddATP, dTTP, dCTP, and dGTP.
Sample Solution